Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS.
J Clin Endocrinol Metab
; 105(3)2020 03 01.
Article
in En
| MEDLINE
| ID: mdl-31720688
ABSTRACT
CONTEXT The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations. OBJECTIVE:
To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3).DESIGN:
LC-MS/MS method development and construction of estrogen reference ranges. SETTINGS Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas.PARTICIPANTS:
Healthy participants aged 3 months to 61 years (n = 1838).RESULTS:
An isotope diluted LC-MS/MS method was developed and validated for measurements of serum E1, E2, and E3. Limits of detections (LODs) were 3 pmol/L (E1), 4 pmol/L (E2), and 12 pmol/L (E3), respectively. This sensitive method made it possible to differentiate between male and female concentration levels of E1 and E2 in children. In girls, E2 levels ranged fromCONCLUSION:
Reference ranges of simultaneous quantification of E1 and E2 by this novel specific and highly sensitive LC-MS/MS method provide an invaluable tool in clinical practice and in future research studies.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sex Factors
/
Chromatography, Liquid
/
Estradiol
/
Estrone
/
Tandem Mass Spectrometry
Type of study:
Diagnostic_studies
Limits:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
/
Middle aged
Language:
En
Journal:
J Clin Endocrinol Metab
Year:
2020
Document type:
Article
Affiliation country:
Dinamarca