Effects of microcystins-LR on genotoxic responses in human intestinal epithelial cells (NCM460).
J Toxicol Environ Health A
; 82(21): 1113-1119, 2019.
Article
in En
| MEDLINE
| ID: mdl-31818208
ABSTRACT
Microcystin-LR (MC-LR), a cyclic heptapeptide toxin produced by cyanobacteria, was found to induce genotoxic actions in various types of cells. Some investigators reported that microcystin-LR acted as tumor initiator in the observed genotoxic action mediated by this cyanotoxin. However, the underlying mechanisms underlying MC-induced DNA damage in the human intestine epithelium cell line (NCM460) are not known. The purpose of this study was to examine the influence of 24 hr exposure to 5 or 10 µM MC-LR on intestinal DNA damage using NCM460 intestine cell line as a model. Data showed that MC-LR increased Olive tail moment (OTM) as evidenced by the comet assay, inhibited protein phosphatase 2A (PP2A) activity, elevated reactive oxygen species levels (ROS) and enhanced γ-H2AX and p-p53 protein expression levels. Results indicated that MC-LR produced intestinal DNA damage by inhibiting PP2A activity, activating p53 protein and subsequently initiating excess generation of ROS. These observations suggest that MC-LR-induced intestinal DNA damage involves a complex series of events that include oxidant stress, PP2A enzymic inhibition and activation of p53 protein.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Damage
/
Epithelial Cells
/
Microcystins
/
Intestinal Mucosa
Limits:
Humans
Language:
En
Journal:
J Toxicol Environ Health A
Journal subject:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Year:
2019
Document type:
Article
Affiliation country:
China