FTIR Microspectroscopic Analysis of the Water Oxidation Reaction in a Single Photosystem II Microcrystal.
J Phys Chem B
; 124(1): 121-127, 2020 01 09.
Article
in En
| MEDLINE
| ID: mdl-31825617
ABSTRACT
Microcrystals of photosystem II (PSII) have recently been used to investigate the intermediate structures of the water oxidizing complex during water oxidation by serial femtosecond crystallography using X-ray free electron lasers. To clarify the water oxidation mechanism, it is crucial to know whether the reaction proceeds properly in the microcrystals. In this work, we monitored the water oxidation reaction in a single PSII microcrystal using Fourier transform infrared (FTIR) microspectroscopy with the transmission method. Flash-induced micro-FTIR difference spectra of S-state transitions in a PSII microcrystal showed features virtually identical to the corresponding spectra previously obtained using the attenuated total reflection method for multiple microcrystals, representing the reactions near the crystal surface, as well as the spectra in solution. This observation indicates that the reaction processes of water oxidation proceed with relatively high efficiencies retaining native intermediate structures in the entire inside of a PSII microcrystal.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Water
/
Spectroscopy, Fourier Transform Infrared
/
Photosystem II Protein Complex
Language:
En
Journal:
J Phys Chem B
Journal subject:
QUIMICA
Year:
2020
Document type:
Article
Affiliation country:
Japón