Statistical optimization and characterization of a biocellulose produced by local Egyptian isolate Komagataeibacter hansenii AS.5.
Int J Biol Macromol
; 144: 198-207, 2020 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-31843613
ABSTRACT
Optimization of the culture parameters used for biocellulose (BC) production by a previously isolated bacterial strain (Komagataeibacter hansenii AS.5) was carried out. The effect of nine culture parameters on BC production was evaluated by implementing the Plackett-Burman design, and the results revealed that, the most significant variables affecting BC production were MgSO4, ethanol, pH and yeast extract. A three-level and four-factor Box-Behnken design was applied to determine the optimum level of each significant variable. According to the results of the Plackett-Burman (PBD) and Box-Behnken designs (BBD), the following medium composition and parameters were calculated to be optimum (g/l) glucose 25, yeast extract 13, MgSO4 0.15, KH2PO4 2, ethanol 7.18 ml/l, pH 5.5, inoclume size 7%, cultivation temperature 20 °C and incubation time 9 days. Characterization of purified BC was performed to determine the network morphology by scanning electron microscopy, crystallinity by X-ray diffraction, chemical structure and functional groups by Fourier-transform infrared spectroscopy, thermal stability by thermogravimetric analysis and mechanical properties such as Young's modulus, tensile strength and elongation at beak % of BC.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cellulose
/
Acetobacteraceae
Language:
En
Journal:
Int J Biol Macromol
Year:
2020
Document type:
Article