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A tRNA-Derived Small RNA Regulates Ribosomal Protein S28 Protein Levels after Translation Initiation in Humans and Mice.
Kim, Hak Kyun; Xu, Jianpeng; Chu, Kirk; Park, Hyesuk; Jang, Hagoon; Li, Pan; Valdmanis, Paul N; Zhang, Qiangfeng Cliff; Kay, Mark A.
Affiliation
  • Kim HK; Department of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA; Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
  • Xu J; Department of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
  • Chu K; Department of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
  • Park H; Department of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
  • Jang H; Department of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
  • Li P; MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Valdmanis PN; Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA.
  • Zhang QC; MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Kay MA; Department of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA. Electronic address: markay@stanford.edu.
Cell Rep ; 29(12): 3816-3824.e4, 2019 12 17.
Article in En | MEDLINE | ID: mdl-31851915
ABSTRACT
tRNA-derived small RNAs (tsRNAs) have been implicated in many cellular processes, yet the detailed mechanisms are not well defined. We previously found that the 3' end of Leu-CAG tRNA-derived small RNA (LeuCAG3'tsRNA) regulates ribosome biogenesis in humans by maintaining ribosomal protein S28 (RPS28) levels. The tsRNA binds to coding (CDS) and non-coding 3' UTR sequence in the RPS28 mRNA, altering its secondary structure and enhancing its translation. Here we report that the functional 3' UTR target site is present in primates while the CDS target site is present in many vertebrates. We establish that this tsRNA also regulates mouse Rps28 translation by interacting with the CDS target site. We further establish that the change in mRNA translation occurred at a post-initiation step in both species. Overall, our results suggest that LeuCAG3'tsRNA might maintain ribosome biogenesis through a conserved gene regulatory mechanism in vertebrates.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ribosomal Proteins / Protein Biosynthesis / RNA, Messenger / RNA, Transfer / Protein Processing, Post-Translational / RNA, Small Untranslated / Leucine Limits: Animals / Humans Language: En Journal: Cell Rep Year: 2019 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ribosomal Proteins / Protein Biosynthesis / RNA, Messenger / RNA, Transfer / Protein Processing, Post-Translational / RNA, Small Untranslated / Leucine Limits: Animals / Humans Language: En Journal: Cell Rep Year: 2019 Document type: Article