Dezocine attenuates the remifentanil-induced postoperative hyperalgesia by inhibition of phosphorylation of CaMKâ
¡α.
Eur J Pharmacol
; 869: 172882, 2020 Feb 15.
Article
in En
| MEDLINE
| ID: mdl-31863769
ABSTRACT
Remifentanil, ultra-short-acting µ-opioid receptor agonist, has the greatest advantage in analgesia but could increase postoperative pain scores and induces postoperative hyperalgesia. Dezocine is a mixed opioid receptor partial agonist/antagonist and has been used for postoperative hyperalgesia management in clinical patients,but the potential molecular mechanism is still unclear. Ca2+/calmodulin-dependent protein kinase â
¡(CaMKâ
¡) has been reported involved in remifentanil-induced hyperalgesia (RIH) in previous studies, but the relationship between CaMKâ
¡ and dezocine in RIH is still unclear. To investigate the mechanism of dezocine in RIH, we used a remifentanil induced postoperative hyperalgesia (RIPH) in incisional pain model of mouse. We subcutaneously infused remifentanil (40 µg/kg) to induce postoperative hyperalgesia. Dezocine (1.5 mg/kg, 3.0 mg/kg, and 6.0 mg/kg) was infused subcutaneously with remifentanil using the apparatus pump for 30 min. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) were used to assess thermal hyperalgesia and mechanical allodynia. Western blotting analysis and immunohistochemistry analysis were used to assess the expression of phosphorylated CaMKâ
¡α (p-CaMKâ
¡α) in somatosensory cortex, hippocampus and spinal cord. Subcutaneous infusion of remifentanil enhanced postoperative pain induced by surgical incision and increased PWTL and PWMT. Dezocine dose-dependently decreased the PWTL and PWMT in RIPH model. Correlating with behavioral effects, dezocine inhibited remifentanil-induced up-regulation of p-CaMKâ
¡α expression in somatosensory cortex, hippocampus and spinal cord. Dezocine could attenuate RIPH by suppressing p-CaMKâ
¡α.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pain, Postoperative
/
Tetrahydronaphthalenes
/
Bridged Bicyclo Compounds, Heterocyclic
/
Calcium-Calmodulin-Dependent Protein Kinase Type 2
/
Hyperalgesia
/
Analgesics, Opioid
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Eur J Pharmacol
Year:
2020
Document type:
Article
Affiliation country:
China