The mimetic assembly of cobalt prot-porphyrin with cyclodextrin dimer and its application for H2O2 detection.

ABSTRACT

A biomimetic assembly of per-O-methylated-cyclodextrin dimer with cobalt proto-porphyrin (CoIII-PPIX@Py2CD) was achieved via covalent linkage between CoIII of CoIII-PPIX and pyridine N of Py2CD (primarily synthesized by the acyl chlorination reaction of two ß-CDs monomers with 3,5-bis (bromomethyl) pyridine). Ultraviolet-visible (UV-vis) and circular dichroism (CD) absorption spectroscopy, and NMR hydrogen spectroscopy (H1-NMR) were adopted to carefully characterize the structure of Py2CD and its functional assembly with CoIII-PPIX. X-ray photoelectron spectroscopy (XPS) was employed to affirm the binding of the as-obtained CoIII-PPIX@Py2CD, whose electrochemical kinetics were extensively studied to validate the feasibility in the catalytic reduction of hydrogen peroxide (H2O2). The developed sensor displayed the wide linear range for H2O2 detection and the low detection limit of 2.47 × 10-7 M. This work sheds some constructive lights on rational design and synthesis of preeminently biomimic carrier and high cost-effectiveness catalyst for (bio)analytical applications.