Prevalence of anti-DFS70 autoantibodies in a Latin American cohort of patients with systemic lupus erythematosus and without autoimmune diseases.
Clin Rheumatol
; 39(7): 2163-2169, 2020 Jul.
Article
in En
| MEDLINE
| ID: mdl-32086623
ABSTRACT
INTRODUCTION/OBJECTIVES:
Anti-dense fine speckled 70 (DFS70) autoantibodies were reported to be more prevalent in healthy individuals than those with autoimmune diseases such as systemic lupus erythematosus (SLE). We determined anti-DFS70 autoantibody prevalence in a Latin American cohort of patients with SLE and healthy individuals.METHODS:
This study included 127 individuals with anti-nuclear antibodies (ANAs; > 1160) suggesting the presence of anti-DFS70, including 64 patients with SLE and 63 healthy controls. The anti-DFS70 autoantibodies were determined by immunoadsorption using NOVA Lite® HEp-2 Select kit with DAPI. Negative fluorescence after adsorption with the DFS70 antigen indicated anti-DFS70 autoantibody positivity.RESULTS:
The presence of anti-DFS70 autoantibodies was confirmed by indirect immunofluorescence in 21 (33.3%) healthy controls and 8 (12.5%) patients with SLE (p = 0.005). Among the anti-DFS70-positive patients with SLE, the most frequent compromise was renal involvement in six cases (75%), 4 patients (37.5%) were positive for anti-Sm, which was the most frequently associated antibody, and one patient (12.5%) was positive for anti-DNA.CONCLUSIONS:
Anti-DFS70 autoantibodies might be considered a biomarker to differentiate patients with SLE from ANA-positive individuals without autoimmune diseases. KEY POINTS ⢠In a Latin American cohort, the anti-DFS70 was higher in individuals without autoimmune diseases compared with that in patients with SLE.⢠The anti-DFS70 might have utility as a biomarker of exclusion in patients with non-specific clinical signs of AARDs.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Antibodies, Antinuclear
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Adaptor Proteins, Signal Transducing
/
Lupus Erythematosus, Systemic
Type of study:
Diagnostic_studies
/
Observational_studies
/
Prevalence_studies
Limits:
Adult
/
Female
/
Humans
/
Male
Country/Region as subject:
America do sul
/
Colombia
Language:
En
Journal:
Clin Rheumatol
Year:
2020
Document type:
Article
Affiliation country:
Colombia