Methylobacterium terricola sp. nov., a gamma radiation-resistant bacterium isolated from gamma ray-irradiated soil.
Int J Syst Evol Microbiol
; 70(4): 2449-2456, 2020 Apr.
Article
in En
| MEDLINE
| ID: mdl-32100693
ABSTRACT
A gamma radiation-resistant and pink-pigmented bacterial strain, designated as 17Sr1-39T, was isolated from a gamma ray-irradiated soil sample collected in the Republic of Korea. Cells were Gram-stain-negative, strictly aerobic, flagellated, asporogenous, rod-shaped and methylotrophic. Results of 16S rRNA gene sequence analysis showed that strain 17Sr1-39T was phylogenetically related to Methylobacterium currus PR1016AT (97.3â%), Methylobacterium aquaticum DSM 16371T (97.2â%), Methylobacterium platani PMB02T (97.0â%), Methylobacterium frigidaeris IER25-16T (96.6â%), Methylobacterium terrae 17Sr1-28T (96.6â%) and Methylobacterium organophilum JCM 2833T (93.4â%). The G+C content calculated based on the genome sequence was 70.4âmol%. The average nucleotide identity and in silico DNA-DNA hybridization values between strain 17Sr1-39T and M. currus, M. aquaticum, M. platani, M. frigidaeris, M. terrae and M. organophilum were 77.3-89.9 and 22-38.2â%, respectively. The predominant fatty acids were summed feature 8 (C18ââ1ω7c and/or C18ââ1ω6c) and summed feature 3 (C16ââ1ω7c and/or C16ââ1ω6c). The predominant quinone was ubiquinone 10 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on the data from phenotypic tests and genotypic differences between strain 17Sr1-39T and its close phylogenetic relatives, strain 17Sr1-39T represented a new species belonging to the genus Methylobacterium, for which the name Methylobacterium terricola sp. nov. (=KACC 52905T=NBRC 112874T) is proposed.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Soil Microbiology
/
Methylobacterium
/
Gamma Rays
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2020
Document type:
Article