Inhibition of Maillard reaction in production of low-molecular-weight chitosan by enzymatic hydrolysis.
Carbohydr Polym
; 236: 116059, 2020 May 15.
Article
in En
| MEDLINE
| ID: mdl-32172874
ABSTRACT
A low-molecular-weight chitosan (LMWC) sample was prepared by enzymatic hydrolysis, and used for investigation of special Maillard reaction products (MRPs) and factors affecting LMWC bioactivities. After undergoing MR, LMWC turned to brown color (termed BLMWC), showed reduction of several indices of rice growth promotion. This alteration of bioactivities was attributable to MRPs in BLMWC. A special MRP, 5-hydroxy-2-pyridine methanol isomer (5-H-2PMIS), was identified by HPLC and LC-MS. Analysis of key factors affecting MR, using this MRP as monitoring target compound and OD420 value, suggested that MR process can be minimized by storing LMWC under vacuum in a dry, low-temperature, neutral-pH environment. Na2SO3 was effective for inhibition of MR, at optimal concentration 0.5 %. Chemical and FTIR analyses showed that Na2SO3-treated sample conformed to the Chinese National Standard of chitosan (GB 29941-2013). Control of MR is essential for application of LMWC in food, pharmaceutical, and other industries.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glycation End Products, Advanced
/
Chitosan
Type of study:
Prognostic_studies
Language:
En
Journal:
Carbohydr Polym
Year:
2020
Document type:
Article