Metal-doped carbon nanoparticles with intrinsic peroxidase-like activity for colorimetric detection of H2O2 and glucose.
J Mater Chem B
; 7(2): 296-304, 2019 01 14.
Article
in En
| MEDLINE
| ID: mdl-32254554
ABSTRACT
Nanomaterial-based enzyme mimics (nanozymes) are attracting increasing attention because of their low production cost, high stability against denaturation, and resistance to high concentrations of substrates. Here, carbon nanoparticles doped with a small amount (<5 mol%) of Pt (denoted as PtCNPs) are synthesized via a facile, cost-effective hydrothermal treatment of p-phenylenediamine (PPD) and K2PtCl4. The obtained PtCNPs possess high aqueous stability, excellent water-dispersibility, and suitable size (â¼15 nm). More interestingly, the PtCNPs exhibit an intrinsic peroxidase-like activity that can quickly catalyze 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2) and produce a blue color. Importantly, since satisfactory catalytic properties were also observed when K2PtCl4 was replaced with CuCl2, NiCl2, or Na2PdCl4 during the synthesis, the PPD- and inorganic metal salt-involved hydrothermal synthetic approach may be developed as a general and simple way to fabricate new nanozymes. Besides, the steady-state kinetics reveals that the PtCNPs have a stronger affinity for TMB and a weaker affinity for H2O2 compared with horseradish peroxidase. On the basis of the color reaction, a colorimetric detection method for H2O2 and glucose has been successfully established with a detection limit of 0.15 and 0.30 µM, respectively. Further, the method has also been successfully applied for glucose detection in human serum samples. To sum up, this work develops a new synthetic method of metal-doped carbon nanomaterials and demonstrates their capability for the sensitive and selective detection of H2O2 and glucose, which may foster the development of new nanozymes for biosensing applications.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Glucose
/
Biosensing Techniques
/
Colorimetry
/
Metal Nanoparticles
/
Glucose
/
Hydrogen Peroxide
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
J Mater Chem B
Year:
2019
Document type:
Article