Dynamic light scattering biosensing based on analyte-induced inhibition of nanoparticle aggregation.
Anal Bioanal Chem
; 412(14): 3423-3431, 2020 May.
Article
in En
| MEDLINE
| ID: mdl-32279165
ABSTRACT
A new approach to direct quantitative detection of small molecules (haptens) by dynamic light scattering biosensing is presented. The proposed technique implements a homogeneous competitive immunoassay and is based on optical detection of specific inhibition of nanoparticle aggregation induced by the analyte in a sample. The technique performance was tested both in buffer and milk for detection of chloramphenicol - antibiotic relevant to food safety diagnostics. Good specificity, sensitivity (LOD in milk is 2.4 ng/ml), precision (4.0 ± 1.2%), ruggedness (8.3%), and 96% recovery in conjunction with a record wide dynamic range (3 orders of magnitude) of the nanosensing technique were demonstrated. Such characteristics complemented by the assay simplicity (no washing step) and a short assay time make the approach attractive for application as an analytical platform for point-of-care and field-oriented diagnostics. Graphical abstract.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
Chloramphenicol
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Metal Nanoparticles
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Antibodies, Immobilized
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Dynamic Light Scattering
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Anti-Bacterial Agents
Limits:
Animals
Language:
En
Journal:
Anal Bioanal Chem
Year:
2020
Document type:
Article
Affiliation country:
Rusia