Long non-coding RNA small nucleolar RNA host gene 6 aggravates pancreatic cancer through upregulation of far upstream element binding protein 1 by sponging microRNA-26a-5p.
Chin Med J (Engl)
; 133(10): 1211-1220, 2020 May 20.
Article
in En
| MEDLINE
| ID: mdl-32433053
ABSTRACT
BACKGROUND:
Pancreatic cancer (PC) is a highly deadly malignancy with few effective therapies. We aimed to unmask the role that long non-coding RNA small nucleolar RNA host gene 6 (SNHG6) plays in PC cells by targeting far upstream element binding protein 1 (FUBP1) via microRNA-26a-5p (miR-26a-5p).METHODS:
SNHG6 expression was predicted by bioinformatics, followed by verification via reverse transcription quantitative polymerase chain reaction. Then, the interactions among SNHG6, miR-26a-5p, and FUBP1 were detected through online software analysis, dual luciferase reporter assay and RNA pull-down. After that, cells were treated with different small interfering RNAs and/or mimic to determine the interactions among SNHG6, miR-26a-5p, and FUBP1 and their roles in PC cells. Finally, the role of SNHG6 in tumor growth in vivo was evaluated by measuring the growth and weight of transplanted tumors in nude mice. A t-test, one-way and two-way analysis of variance were used for data analysis.RESULTS:
Compared with that in normal tissues, SNHG6 was highly expressed in PC tissues (1.00â±â0.05 vs. 1.56â±â0.06, tâ=â16.03, Pâ<â0.001). Compared with that in human pancreatic duct epithelial cells (HPDE6-C7), SNHG6 showed the highest expression in PANC-1 cells (1.00â±â0.06 vs. 3.87â±â0.13, tâ=â34.72, Pâ<â0.001) and the lowest expression in human pancreatic cancer cells (MIAPaCa-2) (1.00â±â0.06 vs. 1.41â±â0.07, tâ=â7.70, Pâ=â0.0015). Compared with the levels in the si-negative control group, SNHG6 (0.97â±â0.05 vs. 0.21â±â0.06, tâ=â16.85, Pâ<â0.001), N-cadherin (0.74â±â0.05 vs. 0.41â±â0.04, tâ=â8.93, Pâ<â0.001), Vimentin (0.55â±â0.04 vs. 0.25â±â0.03, tâ=â10.39, Pâ<â0.001), and ß-catenin (0.62â±â0.05 vs. 0.32â±â0.03, tâ=â8.91, Pâ<â0.001) were decreased, while E-cadherin (0.65â±â0.06 vs. 1.36â±â0.07, tâ=â13.34, Pâ<â0.001) was increased after SNHG6 knockdown or miR-26a-5p overexpression, accompanied by inhibited cell proliferation, migration, and invasion. SNHG6 overexpression exerted the opposite effects. SNHG6 upregulated FUBP1 expression by sponging miR-26a-5p. Silencing SNHG6 blocked the growth of PC in vivo.CONCLUSION:
Silencing SNHG6 might ameliorate PC through inhibition of FUBP1 by sponging miR-26a-5p, thus providing further supporting evidence for its use in PC treatment.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pancreatic Neoplasms
/
RNA-Binding Proteins
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MicroRNAs
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DNA-Binding Proteins
/
RNA, Long Noncoding
Limits:
Animals
/
Humans
Language:
En
Journal:
Chin Med J (Engl)
Year:
2020
Document type:
Article
Affiliation country:
China