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Heat shock transcription factor 2 inhibits intestinal epithelial cell apoptosis through the mitochondrial pathway in ulcerative colitis.
Wang, Wen; Zhang, Fengrui; Li, Xiaoyu; Luo, Juan; Sun, Yang; Wu, Jing; Li, Maojuan; Wen, Yunling; Liang, Hao; Wang, Kunhua; Niu, Junkun; Miao, Yinglei.
Affiliation
  • Wang W; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Zhang F; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Li X; Department of Respiration, The First Hospital of Changsha, Changsha, Hunan, 410005, China.
  • Luo J; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Sun Y; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Wu J; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Li M; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Wen Y; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Liang H; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Wang K; Department of General Surgery, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China.
  • Niu J; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China. Electronic address: drnjk@qq.com.
  • Miao Y; Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, Yunnan, 650032, China. Electronic address: miaoyinglei@yeah.net.
Biochem Biophys Res Commun ; 527(1): 173-179, 2020 06 18.
Article in En | MEDLINE | ID: mdl-32446363
UC is a chronic inflammatory disease of the colonic mucosa and lacks effective treatments because of unclear pathogenesis. Excessive apoptosis of IECs damages the intestinal epithelial barrier and is involved in the progression of UC, but the mechanism is unknown. HSPs are important in maintaining homeostasis and regulate apoptosis through the mitochondrial pathway. In our previous studies, HSF2, an important regulator of HSPs, was highly expressed in UC patients and negatively correlated with inflammation in mice and IECs. Therefore, we hypothesized that HSF2 may protect against intestinal mucositis by regulating the apoptosis of IECs. In this study, a DSS-induced colitis model of hsf2-/- mice was used to explore the relationship between HSF2 and apoptosis in IECs for the first time. The expression of HSF2 increased in the WT + DSS group compared with that in the WT + H2O group. Moreover, the extent of apoptosis was more severe in the KO + DSS group than in the WT + DSS group. The results showed that HSF2 was negatively correlated with apoptosis in vivo. The expression of HSF2 in Caco-2 cells was changed by lentiviral transfection, and the expression of Bax, cytoplasmic Cyto-C, Cleaved Caspase-9 and Cleaved Caspase-3 were negatively correlated with the different levels of HSF2. These results suggest that HSF2 negatively regulates apoptosis of IECs through the mitochondrial pathway. This may be one of the potential mechanisms to explain the protective role of HSF2 in UC.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Colitis, Ulcerative / Apoptosis / Epithelial Cells / Heat-Shock Proteins / Intestinal Mucosa / Mitochondria Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Biochem Biophys Res Commun Year: 2020 Document type: Article Affiliation country: China Country of publication: Estados Unidos

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Colitis, Ulcerative / Apoptosis / Epithelial Cells / Heat-Shock Proteins / Intestinal Mucosa / Mitochondria Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Biochem Biophys Res Commun Year: 2020 Document type: Article Affiliation country: China Country of publication: Estados Unidos