Comparison of neuronal death and expression of TNFα and MCT4 in the gerbil hippocampal CA1 region induced by ischemia/reperfusion under hyperthermia to those under normothermia.
Mol Med Rep
; 22(2): 1044-1052, 2020 08.
Article
in En
| MEDLINE
| ID: mdl-32468005
ABSTRACT
Monocarboxylate transporter 4 (MCT4) is a highcapacity lactate transporter in cells and the alteration in MCT4 expression harms cellular survival. The present study investigated whether hypothermia affects tumor necrosis factorα (TNFα) and MCT4 immunoreactivity in the subfield cornu ammonis 1 (CA1) following cerebral ischemia/reperfusion (IR) in gerbils. Hypothermia was induced for 30 min before and during ischemia. It was found that IRinduced death of pyramidal neurons was markedly augmented and occurred faster under hyperthermia than under normothermia. TNFα immunoreactivity in the pyramidal cells started to increase at 3 h after IR and peaked at 1 day after IR under normothermia. However, in hyperthermic control and sham operated gerbils, TNFα immunoreactivity was significantly increased compared with the normothermic gerbils, and IR under hyperthermia caused a more rapid and significant increase in TNFα immunoreactivity in pyramidal neurons than under normothermia. In addition, in the normothermic gerbils, MCT4 immunoreactivity began to decrease in pyramidal neurons from 3 h after IR and markedly increased at 1 and 2 days after IR. On the other hand, MCT4 immunoreactivity in pyramidal neurons of the hyperthermic gerbils was significantly increased from 3 h after IR, maintained until 1 day after IR and markedly decreased at 2 days after IR. These results indicate that acceleration of IRinduced neuronal death under hyperthermia might be closely associated with early alteration of TNFα and MCT4 protein expression in the gerbil hippocampus after IR.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reperfusion Injury
/
Tumor Necrosis Factor-alpha
/
Cell Death
/
Monocarboxylic Acid Transporters
/
CA1 Region, Hippocampal
/
Hyperthermia
/
Neurons
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mol Med Rep
Year:
2020
Document type:
Article