Role of sodium tetraborate as a cardioprotective or competitive agent: Modulation of hypertrophic intracellular signals.
J Trace Elem Med Biol
; 62: 126569, 2020 Dec.
Article
in En
| MEDLINE
| ID: mdl-32563862
ABSTRACT
Boron is an essential trace element in cellular metabolism; however, the molecular mechanism of boron in the heart is unclear. In this study, we examined the effect of sodium tetraborate (as boron source) as a possible protective agent or competitive inhibitor of cardiac hypertrophy in an in vitro murine model. We evaluated different previously reported sodium tetraborate concentrations and it was found that 13⯵M improves viability without affecting the cellular structure. We demonstrated that cardiomyocytes pretreated with sodium tetraborate prevents cellular damage induced by isoproterenol (cardioprotective effect) by increasing proliferation rate and inhibiting apoptosis. In addition, the reduction of the expression of the α1AR and ß1AR adrenergic receptors as well as Erk1/2 was notable. Consequently, the expression of the early response genes c-myc, c-fos and c-jun was delayed. Also, the expression of GATA-4, NFAT, NKx2.5 and myogenin transcription factors involved in sarcomere synthesis declined. In contrast, cardiomyocytes, when treated simultaneously with sodium tetraborate and isoproterenol, did not increase their size (cytoplasmic gain), but an increase in apoptosis levels was observed; therefore, the proliferation rate was reduced. Although the mRNA levels of α1AR and ß1AR as well as Erk1/2 and Akt1 were low at 24â¯h, their expression increased to 48â¯h. Notably, the mRNA of expression levels of c-myc, c-fos and c-jun were lower than those determined in the control, while the transcription factors GATA-4, MEF2c, Nkx2.5, NFAT and CDk9 were determined in most cells. These results suggest that pretreatment with sodium tetraborate in cardiomyocytes inhibits the hypertrophic effect. However, sodium tetraborate attenuates isoproterenol induced hypertrophy damage in cardiomyocytes when these two compounds are added simultaneously.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Borates
/
Cardiotonic Agents
/
Cardiomegaly
/
Myocytes, Cardiac
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Trace Elem Med Biol
Journal subject:
METABOLISMO
/
SAUDE AMBIENTAL
Year:
2020
Document type:
Article
Affiliation country:
México
Publication country:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY