One-Step Bacterial Artificial Chromosome (BAC) Modification: Preparation of the A Homology Arm (A-Box).
Cold Spring Harb Protoc
; 2020(7): 098103, 2020 07 01.
Article
in En
| MEDLINE
| ID: mdl-32611777
ABSTRACT
The one-step approach to bacterial artificial chromosome (BAC) modification requires that only one homology arm be cloned into the shuttle vector (in the example presented here, we use the "A-box"). The homology arm, which in this case lies upstream of the ATG start codon, is amplified by polymerase chain reaction (PCR) using purified BAC DNA as template. The resulting amplification product is then digested with the appropriate restriction endonuclease to render it suitable for cloning into the shuttle vector.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Bacterial
/
Polymerase Chain Reaction
/
Chromosomes, Artificial, Bacterial
/
Genetic Vectors
Language:
En
Journal:
Cold Spring Harb Protoc
Year:
2020
Document type:
Article