Measurement of the Cell-Body Rotation of Leptospira.
Methods Mol Biol
; 2134: 139-148, 2020.
Article
in En
| MEDLINE
| ID: mdl-32632866
ABSTRACT
Leptospira spp. swim in liquid and crawl on surfaces with two periplasmic flagella. The periplasmic flagella attach to the protoplasmic cylinder via basal rotary motors (flagellar motors) and transform the ends of the cell body into spiral or hook shape. The rotations of the periplasmic flagella are thought to gyrate the cell body and rotate the protoplasmic cylinder for propelling the cell; however, the motility mechanism has not been fully elucidated. Since the motility is a critical virulence factor for pathogenic leptospires, the kinematic insight is valuable to understand the mechanism of infection. This chapter describes microscopic methodologies to measure the motility of Leptospira, focusing on rotation of the helical cell body.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Body
/
Leptospira
/
Microscopy
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2020
Document type:
Article
Affiliation country:
Japón