Gene's co-expression network and experimental validation of molecular markers associated with the drug resistance of gastric cancer.
Biomark Med
; 14(9): 761-773, 2020 06.
Article
in En
| MEDLINE
| ID: mdl-32715733
ABSTRACT
Aim:
Chemotherapy can significantly improve the overall survival rate of patients with gastric cancer; however, so far little is known about the molecular mechanism of resistance to chemotherapy. Therefore, this study was proposed to elucidate molecular markers of resistance to chemotherapeutic agent in gastric cancer. Materials &methods:
Weighted gene co-expression network analyses were performed in gastric cancer cohort. The most relevant genes modules for gastric cancer resistance were selected. Gene oncology function enrichment of genes was conducted. The biological function of resistant genes were identified in vitro. Results &conclusion:
Two resistant hub genes, SPTBN1 and LAMP1, were selected. Experiments showed that downregulation of SPTBN1and LAMP1 proteins significantly enhanced the sensitivity of human gastric cancer cells SGC7901 to 5-FU and cisplatin. Thus, our results provide a baseline about the potential factors of drug resistance in gastric cancer.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stomach Neoplasms
/
Biomarkers, Tumor
/
Drug Resistance, Neoplasm
/
Gene Regulatory Networks
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
Biomark Med
Journal subject:
BIOQUIMICA
/
MEDICINA
Year:
2020
Document type:
Article
Affiliation country:
China