Functional annotation of human long noncoding RNAs via molecular phenotyping.
Genome Res
; 30(7): 1060-1072, 2020 07.
Article
in En
| MEDLINE
| ID: mdl-32718982
ABSTRACT
Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-to-date lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Long Noncoding
Limits:
Humans
Language:
En
Journal:
Genome Res
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2020
Document type:
Article
Affiliation country:
Japón