Effects of thyroid hormone on ovarian cell apoptosis in the rat.
Reprod Fertil Dev
; 32(12): 1060-1066, 2020 Aug.
Article
in En
| MEDLINE
| ID: mdl-32758353
ABSTRACT
Follicle development is a complicated process regulated by thyroid hormone (TH). TH dysregulation is associated with reproductive disorders; however, the mechanism underlying these relationships remains unclear. Glucose-related protein 78 (GRP78) is a well-characterised endoplasmic reticulum stress protein related to ovarian cell apoptosis. To clarify whether GRP78 is regulated by TH and the involvement of GRP78 in follicle development, we established rat models of hypothyroidism and hyperthyroidism and investigated the effects of TH dysregulation on levels of GRP78, C/EBP homologous protein (CHOP) and cleaved caspase-3. TH dysregulation decreased levels of GRP78 and increased those of CHOP and cleaved caspase-3 in both rat models. However, treatment with equine chorionic gonadotrophin reversed these effects, as well as granulosa cell apoptosis induced by TH dysregulation. Together, these results provide evidence that TH dysregulation alters the GRP78 expression profile, triggering the apoptotic signalling pathway, and suggest that GRP78 is a novel mediator of TH in follicle development.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apoptosis
/
Endoplasmic Reticulum Stress
/
Ovarian Follicle
/
Heat-Shock Proteins
/
Hyperthyroidism
/
Hypothyroidism
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Reprod Fertil Dev
Journal subject:
MEDICINA REPRODUTIVA
Year:
2020
Document type:
Article