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Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR.
Dorlass, Erick Gustavo; Monteiro, Cairo Oliveira; Viana, Amanda Oliveira; Soares, Camila Pereira; Machado, Rafael Rahal Guaragna; Thomazelli, Luciano Matsumiya; Araujo, Danielle Bastos; Leal, Fabyano Bruno; Candido, Erika Donizette; Telezynski, Bruna Larotonda; Valério, Camila Araujo; Chalup, Vanessa Nascimento; Mello, Ralyria; Almeida, Flavia Jaqueline; Aguiar, Andressa Simões; Barrientos, Anna Carlotta Mott; Sucupira, Carolina; De Paulis, Milena; Sáfadi, Marco Aurélio Palazzi; Silva, Daniella Gregorio Bonfim Prado; Sodré, Janaina Joice Martins; Soledade, Mariana Pereira; Matos, Samantha Faria; Ferreira, Sabrina Rodrigues; Pinez, Célia Miranda Nunez; Buonafine, Carolina Palamin; Pieroni, Leticia Nery Ferreira; Malta, Fernanda Mello; Santana, Rubia Anita Ferraz; Souza, Eloisa Corrêa; Fock, Ricardo Ambrosio; Pinho, João Renato Rebelo; Ferreira, Luís Carlos Souza; Botosso, Viviane Fongaro; Durigon, Edison Luiz; Oliveira, Danielle Bruna Leal.
Affiliation
  • Dorlass EG; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Monteiro CO; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Viana AO; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Soares CP; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Machado RRG; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Thomazelli LM; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Araujo DB; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Leal FB; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Candido ED; Development and Innovation Center, Laboratory of Virology, Butantan Institute, São Paulo, Brazil.
  • Telezynski BL; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Valério CA; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Chalup VN; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Mello R; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Almeida FJ; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Aguiar AS; Department of Pediatrics, School of Medical Sciences, Santa Casa de Misericórdia de São Paulo, São Paulo, Brazil.
  • Barrientos ACM; Infection Control Service, São Luiz Gonzaga Hospital of Santa Casa de Misericordia of São Paulo, São Paulo, Brazil.
  • Sucupira C; Infection Control Service and Epidemiological Hospital Nucleo, Municipal Children's Hospital Candido Fontoura, São Paulo, Brazil.
  • De Paulis M; Infection Control Service, Municipal Children's Hospital Menino Jesus, São Paulo, Brazil.
  • Sáfadi MAP; Infection Control Service and Epidemiological Hospital Nucleo, Municipal Children's Hospital Candido Fontoura, São Paulo, Brazil.
  • Silva DGBP; Department of Pediatrics, University Hospital, School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Sodré JJM; Department of Pediatrics, School of Medical Sciences, Santa Casa de Misericórdia de São Paulo, São Paulo, Brazil.
  • Soledade MP; Infection Control Service, São Luiz Gonzaga Hospital of Santa Casa de Misericordia of São Paulo, São Paulo, Brazil.
  • Matos SF; Infection Control Service, Municipal Children's Hospital Menino Jesus, São Paulo, Brazil.
  • Ferreira SR; Infection Control Service and Epidemiological Hospital Nucleo, Municipal Children's Hospital Candido Fontoura, São Paulo, Brazil.
  • Pinez CMN; Department of Pediatrics, School of Medical Sciences, Santa Casa de Misericórdia de São Paulo, São Paulo, Brazil.
  • Buonafine CP; Infection Control Service, Municipal Children's Hospital Menino Jesus, São Paulo, Brazil.
  • Pieroni LNF; Clinical Laboratory Division, Pharmacy and Clinical Laboratory Department, University Hospital, University of São Paulo, São Paulo, Brazil.
  • Malta FM; Department of Pediatrics, School of Medical Sciences, Santa Casa de Misericórdia de São Paulo, São Paulo, Brazil.
  • Santana RAF; Infection Control Service, Municipal Children's Hospital Menino Jesus, São Paulo, Brazil.
  • Souza EC; Clinical Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil.
  • Fock RA; Clinical Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil.
  • Pinho JRR; Department of Pediatrics, University Hospital, School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Ferreira LCS; Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
  • Botosso VF; Clinical Laboratory Division, Pharmacy and Clinical Laboratory Department, University Hospital, University of São Paulo, São Paulo, Brazil.
  • Durigon EL; Clinical Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil.
  • Oliveira DBL; LIM03/07 Clinics Hospital, School of Medicine, University of São Paulo, São Paulo, Brazil.
Braz J Microbiol ; 51(3): 1117-1123, 2020 Sep.
Article in En | MEDLINE | ID: mdl-32767275
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organic Chemicals / Pneumonia, Viral / Coronavirus Infections / Real-Time Polymerase Chain Reaction / Fluorescent Dyes / Betacoronavirus Type of study: Diagnostic_studies / Health_economic_evaluation / Prognostic_studies Limits: Adolescent / Adult / Animals / Child / Humans / Middle aged Language: En Journal: Braz J Microbiol Year: 2020 Document type: Article Affiliation country: Brasil
Fulltext
Add to My VHL
Print
XML
PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organic Chemicals / Pneumonia, Viral / Coronavirus Infections / Real-Time Polymerase Chain Reaction / Fluorescent Dyes / Betacoronavirus Type of study: Diagnostic_studies / Health_economic_evaluation / Prognostic_studies Limits: Adolescent / Adult / Animals / Child / Humans / Middle aged Language: En Journal: Braz J Microbiol Year: 2020 Document type: Article Affiliation country: Brasil