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Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia.
Abid, Salma; Ferjani, Sana; El Moussi, Awatef; Ferjani, Asma; Nasr, Mejda; Landolsi, Ichrak; Saidi, Karima; Gharbi, Hanène; Letaief, Hajer; Hechaichi, Aicha; Safer, Mouna; Ben Alaya, Nissaf Bouafif Ep; Boubaker, Ilhem Boutiba-Ben.
Affiliation
  • Abid S; Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia; University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES09, 1007, Tunis, Tunisia.
  • Ferjani S; University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES09, 1007, Tunis, Tunisia. Electronic address: ferjsana@yahoo.fr.
  • El Moussi A; Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia; University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES09, 1007, Tunis, Tunisia.
  • Ferjani A; Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia; University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES09, 1007, Tunis, Tunisia.
  • Nasr M; Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia.
  • Landolsi I; Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia.
  • Saidi K; Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia.
  • Gharbi H; Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia.
  • Letaief H; Ministry of Health, National Observatory of New and Emerging Diseases, 1006, Tunis, Tunisia.
  • Hechaichi A; Ministry of Health, National Observatory of New and Emerging Diseases, 1006, Tunis, Tunisia.
  • Safer M; Ministry of Health, National Observatory of New and Emerging Diseases, 1006, Tunis, Tunisia.
  • Ben Alaya NBE; Ministry of Health, National Observatory of New and Emerging Diseases, 1006, Tunis, Tunisia.
  • Boubaker IB; Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia; University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES09, 1007, Tunis, Tunisia.
Diagn Microbiol Infect Dis ; 98(3): 115125, 2020 Nov.
Article in En | MEDLINE | ID: mdl-32768876
ABSTRACT
The aim of this study is to test a pooling approach for the RT-PCR test to detect low viral loads of SARS-CoV-2. We found that a single positive specimen can still be detected in pools of up to 10. Each laboratory should conduct its own evaluation and validation of pooling protocols according to its specific context.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pneumonia, Viral / Mass Screening / Coronavirus Infections / Clinical Laboratory Techniques / Reverse Transcriptase Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Humans Country/Region as subject: Africa Language: En Journal: Diagn Microbiol Infect Dis Year: 2020 Document type: Article Affiliation country: Túnez
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pneumonia, Viral / Mass Screening / Coronavirus Infections / Clinical Laboratory Techniques / Reverse Transcriptase Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Humans Country/Region as subject: Africa Language: En Journal: Diagn Microbiol Infect Dis Year: 2020 Document type: Article Affiliation country: Túnez