Dual Triple Helix-Aptamer Probes for Mix-and-Read Detecting Antibiotics in Fish and Milk.
J Agric Food Chem
; 68(35): 9524-9529, 2020 Sep 02.
Article
in En
| MEDLINE
| ID: mdl-32786851
ABSTRACT
Antibiotic abuse in agricultural products leads to serious food safety issues. To this end, we proposed a mix-and-read and enzyme-free amplified assay for antibiotics based on a dual triple helix-aptamer probe, potentially applicable for on-site monitoring of antibiotic residues. A dual triple helix-aptamer probe can leverage the response toward target molecules without enzyme-based amplification, rendering it sensitive and robust for profiling target molecules. The proposed assay allowed mix-and-read detection of chloramphenicol with a detection limit of 0.18 nM. Besides, it accommodated for specifically resolving chloramphenicol among other antibiotics. Chloramphenicol residual in aquatic products in fish and milk can be precisely determined. Thus, the aptamer probe deems to enrich the toolbox for managing antibiotic use.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Food Contamination
/
Milk
/
SELEX Aptamer Technique
/
Meat
/
Anti-Bacterial Agents
Type of study:
Evaluation_studies
Limits:
Animals
Language:
En
Journal:
J Agric Food Chem
Year:
2020
Document type:
Article
Affiliation country:
China