Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR.
J Microbiol Methods
; 178: 106072, 2020 Oct 06.
Article
in En
| MEDLINE
| ID: mdl-33031896
ABSTRACT
Carbapenemase Inactivation Method (CIM) is a test to detect presence of the carbapenemase in Gram-negative bacteria. Determination of the carbapenemase production by inactivation of meropenem requires that a zone of control E. coli inhibition be measured approximately 6-24 h after plating. We have modified the CIM test by developing a rapid method which instead measures the growth of E. coli indicator strain ATCC 25922 using real-time PCR, referred to as a nucleic acid testing CIM (natCIM). Our natCIM, therefore reduces the detecting time from 6 to 24 h to approximately 4 h.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Microbiol Methods
Year:
2020
Document type:
Article
Affiliation country:
Estados Unidos