Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression.
Mol Med
; 26(1): 96, 2020 10 27.
Article
in En
| MEDLINE
| ID: mdl-33109073
ABSTRACT
OBJECTIVES:
Kelch repeat and BTB domain-containing protein 8, KBTBD8, has been identified as a female fertility factor. However, there have been no reports on the role of KBTBD8 in the progression of epithelial ovarian cancer, EOC. Our study aimed to address this issue.METHODS:
We first examine KBTBD8 expression in EOC tissues and cells. Next, we performed RNA sequencing to reveal the overall mechanism. Then we investigated the roles of KBTBD8 in the proliferation, migration, and health status of cultured EOC cells. Finally, we employed tumor xenograft models to evaluate the role of KBTBD8 in vivo.RESULTS:
First, KBTBD8 level was significantly higher in EOC tissues and cells. Next, comparative RNA sequencing identified more tumorigenesis-related genes that KBTBD8 might regulate. Then we found that KBTBD8 knockdown significantly decreased EOC cell proliferation, migration, and the activities of multiple tumorigenesis-related kinases. Finally, KBTBD8 knockdown significantly diminished ovarian tumor formation in vivo.CONCLUSION:
Proper KBTBD8 level is essential for the healthy growth of ovarian somatic cells, such as ovarian epithelial cells. Excessive KBTBD8 might be a significant impetus for EOC progression. KBTBD8 reduction greatly inhibits EOC proliferation and migration.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ovarian Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Adaptor Proteins, Signal Transducing
/
Carcinoma, Ovarian Epithelial
Type of study:
Prognostic_studies
Aspects:
Patient_preference
Limits:
Adult
/
Aged
/
Animals
/
Female
/
Humans
/
Middle aged
Language:
En
Journal:
Mol Med
Journal subject:
BIOLOGIA MOLECULAR
Year:
2020
Document type:
Article
Affiliation country:
China