Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation.
STAR Protoc
; 1(2): 100091, 2020 09 18.
Article
in En
| MEDLINE
| ID: mdl-33111123
ABSTRACT
Many studies in systems neuroscience use head-fixation preparations for in vivo experimentation. While head-fixation confers several advantages, one major limitation is the lack of behavioral measures that quantify whole-body movements. Here, we detail a step-by-step protocol for using a novel head-fixation device that measures the forces exerted by head-fixed mice in multiple dimensions. We further detail how this system can be used in conjunction with in vivo electrophysiology and optogenetics to study dopamine neurons in the ventral tegmental area. For complete details on the use and execution of this protocol, please refer to Hughes et al. (2020a, 2020b).
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Restraint, Physical
/
Ventral Tegmental Area
/
Electrophysiology
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2020
Document type:
Article
Affiliation country:
Estados Unidos