Metal-induced G-quadruplex polymorphism for ratiometric and label-free detection of lead pollution in tea.
Food Chem
; 343: 128425, 2021 May 01.
Article
in En
| MEDLINE
| ID: mdl-33127221
ABSTRACT
Lead pollution are critical concerns for food safety and human health. Herein, a ratiometric metal-induced G-quadruplex polymorphism was introduced to construct aptamer probes, enabling label-free and ratiometric detection of lead in tea, thus is promising for on-site detection of lead pollution. The key feature of the aptamer probe is the synergistic utilization of the dual-wavelength fluorescent signal outputs from a G-quadruplex specific dye and a DNA intercalation dye under a single-wavelength excitation, leading to a more stable and reliable recognition of Pb2+ than that of analyses based on single fluorescent reporter. The aptamer probe allowed to a mix-and-read, rapid, cost-effective detection of Pb2+ with high specificity and accuracy. Pb2+ analysis in tap water and tea exhibited good performance with recovery rates of 92.3%-109.0%. The adoption of ratiometric metal-induced G-quadruplex polymorphism would be a compelling design strategy for constructing robust aptasensor, facilitating the translation of aptamer for food safety control.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tea
/
Food Contamination
/
G-Quadruplexes
/
Food Analysis
/
Lead
Type of study:
Diagnostic_studies
Language:
En
Journal:
Food Chem
Year:
2021
Document type:
Article
Affiliation country:
China