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Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: In vitro characterization and in vivo tissue repair.
Oliveira, D M L; Rezende, P S; Barbosa, T C; Andrade, L N; Bani, C; Tavares, D S; da Silva, C F; Chaud, M V; Padilha, F; Cano, A; de Albuquerque Júnior, R L C; Souto, E B; Severino, P.
Affiliation
  • Oliveira DML; University of Tiradentes (Unit), Biotechnological Postgraduate Program, Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil; Institute of Technology and Research (ITP), Nanomedicine and Nanotechnology Laboratory (LNMed), Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil.
  • Rezende PS; University of Tiradentes (Unit), Biotechnological Postgraduate Program, Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil.
  • Barbosa TC; University of Tiradentes (Unit), Biotechnological Postgraduate Program, Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil.
  • Andrade LN; University of Tiradentes (Unit), Biotechnological Postgraduate Program, Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil; Institute of Technology and Research (ITP), Nanomedicine and Nanotechnology Laboratory (LNMed), Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil.
  • Bani C; Department of Morphology, Federal University of Sergipe (UFS), Avenida Marechal Rondon, 49100-000 São Cristovão, Brazil.
  • Tavares DS; Department of Morphology, Federal University of Sergipe (UFS), Avenida Marechal Rondon, 49100-000 São Cristovão, Brazil.
  • da Silva CF; Department of Exact and Earth Sciences, Federal University of São Paulo, Rua Arthur Riedel, 275, Diadema 09972-270, Brazil.
  • Chaud MV; Laboratory of Biomaterials and Nanotechnology for the Development and Evaluation of Bioactive Substances, University of Sorocaba, Rodovia Raposo Tavares Km 925, 18023-000 Sorocaba, São Paulo, Brazil.
  • Padilha F; University of Tiradentes (Unit), Biotechnological Postgraduate Program, Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil; Institute of Technology and Research (ITP), Nanomedicine and Nanotechnology Laboratory (LNMed), Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil.
  • Cano A; Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain; Biomedical Research Networking Centre in Neurodegenerative Diseases (CIBERNED), Madrid, Spain; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saú
  • de Albuquerque Júnior RLC; University of Tiradentes (Unit), Biotechnological Postgraduate Program, Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil; Institute of Technology and Research (ITP), Nanomedicine and Nanotechnology Laboratory (LNMed), Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil.
  • Souto EB; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal; CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: ebsouto@
  • Severino P; University of Tiradentes (Unit), Biotechnological Postgraduate Program, Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil; Institute of Technology and Research (ITP), Nanomedicine and Nanotechnology Laboratory (LNMed), Av Murilo Dantas, 300, 49010-390 Aracaju, Brazil; Tiradentes Institute, 150 Mt Ver
Int J Pharm ; 591: 120001, 2020 Dec 15.
Article in En | MEDLINE | ID: mdl-33141086
ABSTRACT
The aim of this study was to develop and characterize a double layer biomembrane for dual drug delivery to be used for the treatment of wounds. The membrane was composed of chitosan, hydroxypropyl methylcellulose and lidocaine chloride (anesthetic drug) in the first layer, and of sodium alginate-polymyxin B sulphate (antibiotic) nanoparticles as the second layer. A product with excellent thickness (0.01-0.02 mm), adequate mechanical properties with respect to elasticity, stiffness, tension, and compatible pH for lesion application has been successfully obtained. The incorporation of the drugs was confirmed analysing the membrane cross-sections by scanning electron microscopy. A strong interaction between the drugs and the functional groups of respective polymers was confirmed by Fourier-Transform Infrared Spectroscopy, thermal analysis and X-ray diffraction. Microbiological assays showed a high antimicrobial activity when polymyxin B was present to act against the Staphylococcus aureus and Pseudomonas aeruginosa strains. Low cytotoxicity observed in a cell viability colorimetric assay and SEM analysis suggest biocompatibility between the developed biomembrane and the cell culture. The in vivo assay allowed visualizing the healing potential by calculating the wound retraction index and by histological analysis. Our results confirm the effectiveness of the developed innovative biomaterial for tissue repair and regeneration in an animal model.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chitosan / Nanoparticles Limits: Animals Language: En Journal: Int J Pharm Year: 2020 Document type: Article Affiliation country: Brasil
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chitosan / Nanoparticles Limits: Animals Language: En Journal: Int J Pharm Year: 2020 Document type: Article Affiliation country: Brasil