G-CSF shifts erythropoiesis from bone marrow into spleen in the setting of systemic inflammation.
Life Sci Alliance
; 4(1)2021 01.
Article
in En
| MEDLINE
| ID: mdl-33234677
ABSTRACT
The anemia of inflammation is related in part to abnormal erythropoiesis in bone marrow. G-CSF regulates granulopoiesis and is increased during systemic inflammation. Here, we have showed that high levels of G-CSF are associated with repression of bone marrow erythropoiesis and expansion of splenic erythropoiesis in Escherichia coli-infected mice and lipopolysaccharide-treated mice. Under lipopolysaccharide-induced systemic inflammatory conditions in mice, G-CSF neutralization with antibody alleviated the blockage of bone marrow erythropoiesis, prevented the enhancement of splenic erythropoiesis, ameliorated splenomegaly, and reduced the brittleness of spleen. We further demonstrated that after lipopolysaccharide treatment, TLR4-knockout mice display low levels of G-CSF, healthy bone marrow erythropoiesis, almost no stress erythropoiesis in the spleen, and normal size and toughness of spleen. In addition, we found HIF-mediated erythropoietin production is essential for splenic erythropoiesis in the setting of G-CSF-induced suppression of bone marrow erythropoiesis. Our findings identify G-CSF as a critical mediator of inflammation-associated erythropoiesis dysfunction in bone marrow and offer insight into the mechanism of G-CSF-induced splenic erythropoiesis. We provide experimentally significant dimension to the biology of G-CSF.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spleen
/
Bone Marrow
/
Signal Transduction
/
Granulocyte Colony-Stimulating Factor
/
Systemic Inflammatory Response Syndrome
/
Erythropoiesis
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Life Sci Alliance
Year:
2021
Document type:
Article
Affiliation country:
China