Laser Capture Microdissection of Mouse Growth Plate Cartilage.
Methods Mol Biol
; 2245: 105-119, 2021.
Article
in En
| MEDLINE
| ID: mdl-33315198
ABSTRACT
The ability to identify, isolate, and study pure populations of cells is critical for understanding normal physiology in organs and tissues, which involves spatial regulation of signaling pathways and interactions between cells with different functions, expression profiles, and lineages. Here, we focus on assessing the growth plate cartilage, composed of multiple functionally and histologically distinct zones, to investigate temporally and spatially dependent gene expression differences. In this chapter, we describe the method of laser capture microdissection to isolate chondrocytes from different zones of differentiation in the mouse growth plate cartilage for RNA isolation, and subsequent downstream applications, such as RNA-sequencing and quantitative real-time PCR. We also provide an assessment of different factors contributing to the integrity of the isolated RNA, such as staining methods and procedures in RNA isolation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cartilage, Articular
/
Laser Capture Microdissection
/
Growth Plate
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2021
Document type:
Article
Affiliation country:
Estados Unidos