Label-free DNAzyme assays for dually amplified and one-pot detection of lead pollution.
J Hazard Mater
; 406: 124790, 2021 03 15.
Article
in En
| MEDLINE
| ID: mdl-33316668
ABSTRACT
Lead pollution in water and soil often transfers to food, advocating tools for on-site detection of lead pollution to ensure both environmental and food safety. We proposed a label-free, dually amplified and homogeneous DNAzyme assay for sensitive and one-pot detection of lead pollution. Instead of using chemically modified DNA substrate, a structure-response digestion process was introduced to monitor Pb2+ presence-induced cleavage process of unlabeled substrate, further amplifying the response signals and eliminating the use of labeled DNA probes. The DNAzyme assay allowed to detect Pb2+ as low as 0.12 nM and endued a dynamic range from 0.1 nM to 30 nM. In addition, it can specifically identify Pb2+ among other metal ions. We demonstrated that the DNAzyme assay can precisely detect Pb2+ in tap water, milk and fish. Thus, the DNAzyme assay is promising for on-site monitoring lead pollution risk and ensuring environmental and food safety.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
DNA, Catalytic
/
Environmental Pollutants
/
Lead
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Hazard Mater
Journal subject:
SAUDE AMBIENTAL
Year:
2021
Document type:
Article
Affiliation country:
China