Biochemical characterization of Nostoc sp. exopolysaccharides and evaluation of potential use in wound healing.

ABSTRACT

Exopolysaccharides (EPS) produced by cyanobacteria are complex biomolecules of anionic nature with potential biomedical applications. In this study, the EPS produced by the Nostoc sp. strains PCC7936 and PCC7413 were characterized and evaluated as a biomaterial for new wound dressings. The addition of acetate ions to the culture medium slightly stimulated EPS production, achieving 1463.1 ± 16.0 mgL-1 (PCC7413) and 1372.1 ± 29.0 mgL-1 (PCC7936). Both EPS presented nine monosaccharide residues and a MW > 1000 kDa. The acetate addition changed the monosaccharide molar percentages. FTIR and DLS results confirmed the anionic nature and the presence of sulfate groups in both EPS, which are determinant features for biomedical applications. Both EPS at 1%(w/v) formed gels in the presence of 0.4%(w/v) FeCl3. Results obtained for MTT assay and wound healing in vitro scratch assay revealed hydrogels biocompatibility and ability to promote fibroblast migration and proliferation that was greater in PCC7936. The Nostoc EPS hydrogels presented promising properties to be applied in the treatment of skin injuries.