A quantitative medium-throughput assay to measure Caenorhabditis elegans development and reproduction.
STAR Protoc
; 1(3): 100224, 2020 12 18.
Article
in En
| MEDLINE
| ID: mdl-33377116
ABSTRACT
Growth and offspring count are two commonly determined toxicological endpoints for chemical- or gene-induced developmental and reproductive effects in Caenorhabditis elegans. Here, we present a protocol for a 96 h, medium-throughput assay, assessing both endpoints quantitatively within an automated framework using open-source software. The assay utilizes whole 96-well fluorescence images taken with a high-content screening system. Alternatively, conventional fluorescence images can also be utilized with only a few adjustments. For complete details on the use and execution of this protocol, please refer to Wittkowski et al. (2019).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reproduction
/
Biological Assay
/
High-Throughput Screening Assays
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2020
Document type:
Article
Affiliation country:
Alemania