Different roles of Numb-p72 and Numb-p65 on the trafficking of metabotropic glutamate receptor 5.
Mol Biol Rep
; 48(1): 595-600, 2021 Jan.
Article
in En
| MEDLINE
| ID: mdl-33394235
ABSTRACT
We previously reported that Numb, a protein localized to clathrin-coated vesicles, regulates the membrane expression of metabotropic glutamate receptor 5 (mGluR5) and is critical to social behaviors. However, the distinct actions of Numb isoforms on mGluR5 have not been investigated. Here, we showed that the expression patterns of Numb-p72 and Numb-p65, two important isoforms of Numb, were distinct in HEK293T cells. Numb-p72, but not Numb-p65, bound to mGluR5α, and enhanced mGluR5 membrane expression by inhibiting its internalization. Our results suggest that a complete structure is required for Numb to bind to mGluR5 and to modulate mGluR5 trafficking.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Transport
/
Receptor, Metabotropic Glutamate 5
/
Membrane Proteins
/
Nerve Tissue Proteins
Limits:
Humans
Language:
En
Journal:
Mol Biol Rep
Year:
2021
Document type:
Article