Your browser doesn't support javascript.
loading
Identifying the effect of vancomycin on health care-associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media.
Rajput, Akanksha; Poudel, Saugat; Tsunemoto, Hannah; Meehan, Michael; Szubin, Richard; Olson, Connor A; Seif, Yara; Lamsa, Anne; Dillon, Nicholas; Vrbanac, Alison; Sugie, Joseph; Dahesh, Samira; Monk, Jonathan M; Dorrestein, Pieter C; Knight, Rob; Pogliano, Joe; Nizet, Victor; Feist, Adam M; Palsson, Bernhard O.
Affiliation
  • Rajput A; Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Poudel S; Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Tsunemoto H; Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Meehan M; Collaborative Mass Spectrometry Innovation Center, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Szubin R; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Olson CA; Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Seif Y; Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Lamsa A; Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Dillon N; Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Vrbanac A; Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92023, USA.
  • Sugie J; Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Dahesh S; Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92023, USA.
  • Monk JM; Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Dorrestein PC; Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Knight R; Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92023, USA.
  • Pogliano J; Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Nizet V; Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Feist AM; Collaborative Mass Spectrometry Innovation Center, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
  • Palsson BO; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Gigascience ; 10(1)2021 01 09.
Article in En | MEDLINE | ID: mdl-33420779
ABSTRACT

BACKGROUND:

The evolving antibiotic-resistant behavior of health care-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more.

FINDINGS:

The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments.

CONCLUSIONS:

The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staphylococcal Infections / Methicillin-Resistant Staphylococcus aureus Type of study: Prognostic_studies / Risk_factors_studies Limits: Humans Language: En Journal: Gigascience Year: 2021 Document type: Article Affiliation country: Estados Unidos
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staphylococcal Infections / Methicillin-Resistant Staphylococcus aureus Type of study: Prognostic_studies / Risk_factors_studies Limits: Humans Language: En Journal: Gigascience Year: 2021 Document type: Article Affiliation country: Estados Unidos