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A mycovirus modulates the endophytic and pathogenic traits of a plant associated fungus.
Zhou, Lingling; Li, Xuepei; Kotta-Loizou, Ioly; Dong, Kaili; Li, Shifang; Ni, Dejiang; Hong, Ni; Wang, Guoping; Xu, Wenxing.
Affiliation
  • Zhou L; Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
  • Li X; State Key Laboratory of Agricultural Microbiology, 430070, Wuhan, Hubei, China.
  • Kotta-Loizou I; Key Laboratory of Plant Pathology of Hubei Province, 430070, Wuhan, Hubei, China.
  • Dong K; Key Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, 430070, Wuhan, Hubei, China.
  • Li S; College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, Hubei, China.
  • Ni D; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), 571101, Haikou, Hainan, China.
  • Hong N; Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
  • Wang G; State Key Laboratory of Agricultural Microbiology, 430070, Wuhan, Hubei, China.
  • Xu W; Key Laboratory of Plant Pathology of Hubei Province, 430070, Wuhan, Hubei, China.
ISME J ; 15(7): 1893-1906, 2021 07.
Article in En | MEDLINE | ID: mdl-33531623
Fungi are generally thought to live in host plants with a single lifestyle, being parasitism, commensalism, or mutualism. The former, known as phytopathogenic fungi, cause various plant diseases that result in significant losses every year; while the latter, such as endophytic fungi, can confer fitness to the host plants. It is unclear whether biological factors can modulate the parasitic and mutualistic traits of a fungus. In this study, we isolated and characterized a mycovirus from an endophytic strain of the fungus Pestalotiopsis theae, a pathogen of tea (Camellia sinensis). Based on molecular analysis, we tentatively designated the mycovirus as Pestalotiopsis theae chrysovirus-1 (PtCV1), a novel member of the family Chrysoviridae, genus Alphachrysovirus. PtCV1 has four double-stranded (ds) RNAs as its genome, ranging from 0.9 to 3.4 kbp in size, encapsidated in isometric particles. PtCV1 significantly reduced the growth rates of its host fungus in vitro (ANOVA; P-value < 0.001) and abolished its virulence in planta (ANOVA; P-value < 0.001), converting its host fungus to a non-pathogenic endophyte on tea leaves, while PtCV1-free isolates were highly virulent. Moreover, the presence of PtCV1 conferred high resistance to the host plants against the virulent P. theae strains. Here we report a mycovirus that modulates endophytic and phytopathogenic fungal traits and provides an alternative approach to biological control of plant diseases caused by fungi.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA Viruses / Fungal Viruses Type of study: Risk_factors_studies Language: En Journal: ISME J Journal subject: MICROBIOLOGIA / SAUDE AMBIENTAL Year: 2021 Document type: Article Affiliation country: China Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA Viruses / Fungal Viruses Type of study: Risk_factors_studies Language: En Journal: ISME J Journal subject: MICROBIOLOGIA / SAUDE AMBIENTAL Year: 2021 Document type: Article Affiliation country: China Country of publication: Reino Unido