NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax.
Molecules
; 26(3)2021 Feb 02.
Article
in En
| MEDLINE
| ID: mdl-33540754
ABSTRACT
Lignans, phenolic plant secondary metabolites, are derived from the phenylpropanoid biosynthetic pathway. Although, being investigated for their health benefits in terms of antioxidant, antitumor, anti-inflammatory and antiviral properties, the role of these molecules in plants remains incompletely elucidated; a potential role in stress response mechanisms has been, however, proposed. In this study, a non-targeted metabolomic analysis of the roots, stems, and leaves of wild-type and PLR1-RNAi transgenic flax, devoid of (+) secoisolariciresinol diglucoside ((+) SDG)-the main flaxseed lignan, was performed using 1H-NMR and LC-MS, in order to obtain further insight into the involvement of lignan in the response of plant to osmotic stress. Results showed that wild-type and lignan-deficient flax plants have different metabolic responses after being exposed to osmotic stress conditions, but they both showed the capacity to induce an adaptive response to osmotic stress. These findings suggest the indirect involvement of lignans in osmotic stress response.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osmotic Pressure
/
Mass Spectrometry
/
Magnetic Resonance Spectroscopy
/
Chromatography, Liquid
/
Lignans
/
Flax
/
Metabolomics
Language:
En
Journal:
Molecules
Journal subject:
BIOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
Francia