[Gene cloning, heterologous expression and activity identification of latroeggtoxin-â
¥].
Sheng Wu Gong Cheng Xue Bao
; 37(2): 635-645, 2021 Feb 25.
Article
in Zh
| MEDLINE
| ID: mdl-33645161
ABSTRACT
One of the distinct characters of Latrodectus tredecimguttatus is that its toxic components exist not only in the venomous glands, but also in the tissues outside the venomous glands and even in the eggs. Investigation on the toxins outside the venomous glands can deepen our understanding of spider toxins and discover new lead molecules with important application prospects. In order to explore the low-abundance proteinaceous toxins in the L. tredecimguttatus eggs, we used bioinformatic strategies to mine a gene sequence encoding a peptide toxin from the transcriptome of L. tredecimguttatus eggs, and then heterologously expressed the gene successfully with a 3'-RACE combined with nest PCR strategy. Biological activity analyses indicated that the expressed peptide toxin, named latroeggtoxin-â
¥ (LETX-â
¥), could inhibit Na⺠channel currents in ND7/23 cells and promote dopamine release from PC12 cells, without obvious toxicity against Periplaneta americana and bacteria as well as fungi including Staphylococcus aureus and Candida albicans, demonstrating that LETX-â
¥ is a mammal-specific neurotoxin with a potential application prospect in development of the tool reagents for neurobiological study and the drugs for treating related diseases.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spider Venoms
/
Black Widow Spider
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Sheng Wu Gong Cheng Xue Bao
Journal subject:
BIOTECNOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
China