A colorimetric aptasensor for the simple and rapid detection of human papillomavirus type 16 L1 proteins.
Analyst
; 146(8): 2712-2717, 2021 Apr 26.
Article
in En
| MEDLINE
| ID: mdl-33688885
ABSTRACT
In this study, a novel colorimetric aptasensor was developed for the rapid detection and visual screening of HPV16 L1 proteins using gold nanoparticles (AuNPs) and an RNA aptamer against HPV16 L1 protein (APTHPV16 L1). The AuNP-APTHPV16 L1 conjugates could be aggregated by the addition of a salt in the presence of HPV16 L1 proteins at the ppb level. At the same time, the surface plasma resonance absorption peaks of AuNPs shifted to a short wavelength, and an observable change in color from red to blue occurred. The relative absorbance (Ablank - Asample/Ablank) at 520 nm exhibited a stable response to HPV16 L1 proteins over a concentration range from 9.6 to 201.6 ng mL-1. The visual detection limit of HPV16 L1 proteins was found to be 9.6 ng mL-1. Finally, the proposed colorimetric aptasensor was successfully applied for the rapid and effective detection of HPV16 L1 proteins in clinical samples and vaccine samples. The validity and reliability of the proposed colorimetric aptasensor were verified by the enzyme-linked immunosorbent assay method. The proposed colorimetric aptasensor provided a promising indicator for screening and quantitative detection of HPV16 L1 proteins in clinical samples.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
Oncogene Proteins, Viral
/
Capsid Proteins
/
Aptamers, Nucleotide
/
Alphapapillomavirus
/
Metal Nanoparticles
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Analyst
Year:
2021
Document type:
Article