Analysis of reactive astrogliosis in mouse brain using in situ hybridization combined with immunohistochemistry.
STAR Protoc
; 2(1): 100375, 2021 03 19.
Article
in En
| MEDLINE
| ID: mdl-33718893
ABSTRACT
Reactive astrogliosis is characterized by a profound change in astrocyte phenotype in response to all CNS injuries. Here, we present a revised in situ hybridization and immunohistochemistry (IHC) protocol to label the reactive astrocytes in the mouse brain. Several approaches for quantifying astrocyte reactivity lacked sensitivity to discriminate across the spectrum. We optimized in situ hybridization followed by IHC. We provide a staining protocol for quantitative measures of astrocyte reactivity as an independent confirmation of the magnitude of reactive gliosis. For complete details on the use and execution of this protocol, please refer to Muraleedharan et al. (2020).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunohistochemistry
/
In Situ Hybridization
/
Gliosis
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2021
Document type:
Article
Affiliation country:
Estados Unidos