Phytoremediation of multiple persistent pollutants co-contaminated soil by HhSSB transformed plant.
Environ Res
; 197: 110959, 2021 06.
Article
in En
| MEDLINE
| ID: mdl-33722526
ABSTRACT
The high toxicity of persistent pollutants limits the phytoremediation of pollutants-contaminated soil. In this study, heterologous expressing Halorhodospira halophila single-stranded DNA binding protein gene (HhSSB) improves tolerance to 2,4,6-trinitrotoluene (TNT), 2,4,6-trichlorophenol (2,4,6-TCP), and thiocyanate (SCN-) in A. thaliana and tall fescue (Festuca arundinacea). The HhSSB transformed Arabidopsis, and tall fescue also exhibited enhanced phytoremediation of TNT, 2,4,6-TCP, and SCN- separately contaminated soil and co-contaminated soil compared to control plants. TNT assay was selected to explore the mechanism of how HhSSB enhances the phytoremediation of persistent pollutants. Our result indicates that HhSSB enhances the phytoremediation of TNT by enhancing the transformation of TNT in Arabidopsis. Moreover, transcriptomics and comet analysis revealed that HhSSB improves TNT tolerance through three pathways strengthening the defense system, enhancing the ROS scavenging system, and reducing DNA damage. These results presented here would be particularly useful for further studies in the remediation of soil contaminated by organic and inorganic pollutants.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Soil Pollutants
/
Environmental Pollutants
Language:
En
Journal:
Environ Res
Year:
2021
Document type:
Article
Affiliation country:
China