Sensitive Immunoassay Based on Biocompatible and Robust Silica-Coated Cd-Free InP-Based Quantum Dots.
Inorg Chem
; 60(9): 6503-6513, 2021 May 03.
Article
in En
| MEDLINE
| ID: mdl-33847486
ABSTRACT
Low-toxic InP quantum dots (QDs) as an ideal candidate for Cd-based QDs have tremendous potential for next-generation commercial display and biological detection applications. However, the progress in biological detection is still far behind that of the Cd-based QDs. This is mainly because the InP-based QDs are of inferior stability and photoluminescence quantum yield (PL QY) in aqueous solution. Here, PL QY of 65% and excellent stability of InP/GaP/ZnS QD@SiO2 nanoparticles have been successfully synthesized via a silica coating method. The containing thiol-capped hydrophobic InP/GaP/ZnS QDs were pre-silanized with waterless, ammonia-free hydrolysis tetraethyl orthosilicate, and subsequently, an outer silica shell was generated in the reverse microemulsion. The corresponding QD-based fluorescence-linked immunosorbent assay exhibits a high sensitivity of 0.9 ng mL-1 for C-reactive protein and the broad detection range of 1-1000 ng mL-1, which was close to that of the state-of-the-art Cd-based QD@SiO2 nanoparticles and had the highest sensitivity of Cd-free QDs so far. This work provides a very successful silica coating method for the containing thiol-capped hydrophobic QDs and the QDs highly sensitive to water and oxygen, and the obtained InP/GaP/ZnS QD@SiO2 nanoparticles were considered as the robust, biocompatible, and promising Cd-free fluorescent labels for the further ultra-sensitive detection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphines
/
Biocompatible Materials
/
Enzyme-Linked Immunosorbent Assay
/
Fluorescent Antibody Technique
/
Silicon Dioxide
/
Quantum Dots
/
Indium
Type of study:
Diagnostic_studies
Language:
En
Journal:
Inorg Chem
Year:
2021
Document type:
Article