Identification and distribution of novel badnaviral sequences integrated in the genome of cacao (Theobroma cacao).
Sci Rep
; 11(1): 8270, 2021 04 15.
Article
in En
| MEDLINE
| ID: mdl-33859254
ABSTRACT
Theobroma cacao is one of the most economically important tropical trees, being the source of chocolate. As part of an ongoing study to understand the diversity of the badnavirus complex, responsible for the cacao swollen shoot virus disease in West Africa, evidence was found recently of virus-like sequences in asymptomatic cacao plants. The present study exploited the wealth of genomic resources in this crop, and combined bioinformatic, molecular, and genetic approaches to report for the first time the presence of integrated badnaviral sequences in most of the cacao genetic groups. These sequences, which we propose to name eTcBV for endogenous T. cacao bacilliform virus, varied in type with each predominating in a specific genetic group. A diagnostic multiplex PCR method was developed to identify the homozygous or hemizygous condition of one specific insert, which was inherited as a single Mendelian trait. These data suggest that these integration events occurred before or during the species diversification in Central and South America, and prior to its cultivation in other regions. Such evidence of integrated sequences is relevant to the management of cacao quarantine facilities and may also aid novel methods to reduce the impact of such viruses in this crop.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Diseases
/
Cacao
/
Genome, Plant
/
Crops, Agricultural
/
Badnavirus
Type of study:
Diagnostic_studies
Country/Region as subject:
Africa
Language:
En
Journal:
Sci Rep
Year:
2021
Document type:
Article
Affiliation country:
Francia