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Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo.
Tan, Tao; Wu, Jun; Si, Chenyang; Dai, Shaoxing; Zhang, Youyue; Sun, Nianqin; Zhang, E; Shao, Honglian; Si, Wei; Yang, Pengpeng; Wang, Hong; Chen, Zhenzhen; Zhu, Ran; Kang, Yu; Hernandez-Benitez, Reyna; Martinez Martinez, Llanos; Nuñez Delicado, Estrella; Berggren, W Travis; Schwarz, May; Ai, Zongyong; Li, Tianqing; Rodriguez Esteban, Concepcion; Ji, Weizhi; Niu, Yuyu; Izpisua Belmonte, Juan Carlos.
Affiliation
  • Tan T; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: tant@lpbr.cn.
  • Wu J; Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address: jun2.wu@utsouthwestern.edu.
  • Si C; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Dai S; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Zhang Y; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Sun N; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Zhang E; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Shao H; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Si W; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Yang P; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Wang H; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Chen Z; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Zhu R; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Kang Y; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Hernandez-Benitez R; Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
  • Martinez Martinez L; Universidad Católica San Antonio de Murcia (UCAM), Campus de los Jerónimos, No 135 12, Guadalupe 30107, Spain.
  • Nuñez Delicado E; Universidad Católica San Antonio de Murcia (UCAM), Campus de los Jerónimos, No 135 12, Guadalupe 30107, Spain.
  • Berggren WT; Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
  • Schwarz M; Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
  • Ai Z; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Li T; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
  • Rodriguez Esteban C; Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
  • Ji W; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: wji@lpbr.cn.
  • Niu Y; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: niuyy@lpbr.cn.
  • Izpisua Belmonte JC; Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address: belmonte@salk.edu.
Cell ; 184(8): 2020-2032.e14, 2021 04 15.
Article in En | MEDLINE | ID: mdl-33861963
ABSTRACT
Interspecies chimera formation with human pluripotent stem cells (hPSCs) represents a necessary alternative to evaluate hPSC pluripotency in vivo and might constitute a promising strategy for various regenerative medicine applications, including the generation of organs and tissues for transplantation. Studies using mouse and pig embryos suggest that hPSCs do not robustly contribute to chimera formation in species evolutionarily distant to humans. We studied the chimeric competency of human extended pluripotent stem cells (hEPSCs) in cynomolgus monkey (Macaca fascicularis) embryos cultured ex vivo. We demonstrate that hEPSCs survived, proliferated, and generated several peri- and early post-implantation cell lineages inside monkey embryos. We also uncovered signaling events underlying interspecific crosstalk that may help shape the unique developmental trajectories of human and monkey cells within chimeric embryos. These results may help to better understand early human development and primate evolution and develop strategies to improve human chimerism in evolutionarily distant species.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pluripotent Stem Cells / Chimerism / Embryo, Mammalian Limits: Animals / Female / Humans Language: En Journal: Cell Year: 2021 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pluripotent Stem Cells / Chimerism / Embryo, Mammalian Limits: Animals / Female / Humans Language: En Journal: Cell Year: 2021 Document type: Article