Your browser doesn't support javascript.
loading
Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria.
Strässle, Marina; Laloli, Laura; Gultom, Mitra; V'kovski, Philip; Stoffel, Michael H; Crespo Pomar, Silvia; Chanfon Bätzner, Astrid; Ebert, Nadine; Labroussaa, Fabien; Dijkman, Ronald; Jores, Joerg; Thiel, Volker.
Affiliation
  • Strässle M; Institute of Virology and Immunology (IVI), Bern, Switzerland; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsu
  • Laloli L; Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland; Institute for Infectious Diseases, University of
  • Gultom M; Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland; Institute for Infectious Diseases, University of
  • V'kovski P; Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Stoffel MH; Division of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, Länggass-Str. 120, PO Box 3001, Bern, Switzerland.
  • Crespo Pomar S; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland.
  • Chanfon Bätzner A; Institute of Animal Pathology (COMPATH), Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland.
  • Ebert N; Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Labroussaa F; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland.
  • Dijkman R; Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
  • Jores J; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland. Electronic address: joerg.jores@vetsuisse.unibe.ch.
  • Thiel V; Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Vet Microbiol ; 257: 109067, 2021 Jun.
Article in En | MEDLINE | ID: mdl-33862331
ABSTRACT
Respiratory diseases negatively impact the global goat industry, but are understudied. There is a shortage of established and biological relevant in vitro or ex vivo assays to study caprine respiratory infections. Here, we describe the establishment of an in vitro system based on well-differentiated caprine airway epithelial cell (AEC) cultures grown under air liquid interface conditions as an experimental platform to study caprine respiratory pathogens. The functional differentiation of the AEC cultures was monitored and confirmed by light and immunofluorescence microscopy, scanning electron microscopy and examination of histological sections. We validated the functionality of the platform by studying Influenza D Virus (IDV) infection and Mycoplasma mycoides subsp. capri (Mmc) colonization over 5 days, including monitoring of infectious agents by titration and qPCR as well as colour changing units, respectively. The inoculation of caprine AEC cultures with IDV showed that efficient viral replication takes place, and revealed that IDV has a marked cell tropism for ciliated cells. Furthermore, AEC cultures were successfully infected with Mmc using a multiplicity of infection of 0.1 and colonization was monitored over several days. Altogether, these results demonstrate that our newly-established caprine AEC cultures can be used to investigate host-pathogen interactions of caprine respiratory pathogens.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Respiratory System / Cell Culture Techniques / Respiratory Mucosa / Epithelial Cells Limits: Animals Language: En Journal: Vet Microbiol Year: 2021 Document type: Article
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Respiratory System / Cell Culture Techniques / Respiratory Mucosa / Epithelial Cells Limits: Animals Language: En Journal: Vet Microbiol Year: 2021 Document type: Article