Your browser doesn't support javascript.
loading
Structural characterization of Alpiniae oxyphyllae fructus polysaccharide 2 and its activation effects on RAW264.7 macrophages.
Yang, Xin; Zhou, Shiwei; Li, Hong; An, Jiangwen; Li, Chengheng; Zhou, Ruigang; Teng, Ling; Zhu, Yongjian; Liao, Suya; Yang, Yuhui; Chen, Huricha; Chen, Yun.
Affiliation
  • Yang X; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Zhou S; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Li H; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • An J; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Li C; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Zhou R; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Teng L; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Zhu Y; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Liao S; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Yang Y; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Chen H; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
  • Chen Y; Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China. Electronic address: ychen@hainanu.edu.cn.
Int Immunopharmacol ; 97: 107708, 2021 Aug.
Article in En | MEDLINE | ID: mdl-33915496
ABSTRACT
Polysaccharides are important components of Alpiniae oxyphyllae fructus that have been shown to exhibit significant immunomodulatory activity in our previous study. However, whether and how A. oxyphyllae fructus polysaccharides (AOFP) affect macrophages has not been determined. To further study the immunomodulatory activity of AOFP, the effect of AOFP on RAW264.7 cell activation was investigated in the present work. The results showed that AOFP2 significantly increased the phagocytic activity of RAW264.7 macrophages. AOFP2 promoted the secretion of TNF-α, IL-6, IL-10, TGF-ß, NO and iNOS and enhanced the Th2-type immune response via its activation effect on macrophages. Additionally, the structure of AOFP2 was characterized in the present study, as the structural features of polysaccharides determine their biological activities. AOFP2 was only composed of glucose, exhibiting an average molecular weight of 44.3 kDa. Furthermore, the infrared spectroscopy, methylation and nuclear magnetic resonance results indicated that AOFP2 consisted of â†’ 4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1 â†’ and T-α-Glcp.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polysaccharides / Plant Extracts / Alpinia / Immunologic Factors / Macrophages Limits: Animals Language: En Journal: Int Immunopharmacol Journal subject: ALERGIA E IMUNOLOGIA / FARMACOLOGIA Year: 2021 Document type: Article
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polysaccharides / Plant Extracts / Alpinia / Immunologic Factors / Macrophages Limits: Animals Language: En Journal: Int Immunopharmacol Journal subject: ALERGIA E IMUNOLOGIA / FARMACOLOGIA Year: 2021 Document type: Article