MicroRNAs in the aqueous humor of patients with different types of glaucoma.
Graefes Arch Clin Exp Ophthalmol
; 259(8): 2337-2349, 2021 Aug.
Article
in En
| MEDLINE
| ID: mdl-33929592
ABSTRACT
PURPOSE:
The aim of the study was to compare the frequency and the level of expression of selected miRNAs in the aqueous humor of patients with various types of glaucoma.METHODS:
The studied group consisted of 42 patients with glaucoma 19 with primary open-angle glaucoma (POAG), 14 with pseudoexfoliation glaucoma (PEXG), 9 with primary angle closure glaucoma (PACG), and the control group of 36 patients with senile cataract without glaucoma. The real-time polymerase chain reaction method was used to analyze the expression of miRNAs.RESULTS:
There were no significant differences in the frequency and the level of miRNA expression between various types of glaucoma. There was a tendency for hsa-miR-6722-3p and hsa-miR-184 to be expressed more frequently in PEXG and hsa-miR-1260b in POAG. The expression levels of hsa-miR-1260b and hsa-miR-6515-3p were correlated with age in POAG. Target annotation and functional analyses showed that genes targeted by the most frequently expressed miRNAs (hsa-miR-1202, -1260b, -184, -187-5p, -6515-3p, -6722-3p, and hsa-mir-4634) are involved mainly in response to hypoxia, cardiovascular system development, and apoptosis.CONCLUSION:
Hsa-miR-1260b was the most abundantly expressed among studied miRNAs and may be a potential biomarker of clinical status in PEXG and PACG.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glaucoma
/
Glaucoma, Open-Angle
/
Exfoliation Syndrome
/
MicroRNAs
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Graefes Arch Clin Exp Ophthalmol
Year:
2021
Document type:
Article
Affiliation country:
Polonia