vgrG is separately transcribed from hcp in T6SS orphan clusters and is under the regulation of IHF and HapR.
Biochem Biophys Res Commun
; 559: 15-20, 2021 06 25.
Article
in En
| MEDLINE
| ID: mdl-33932896
ABSTRACT
V. cholerae, the causative agent of cholera epidemic, and V. fluvialis, the emerging foodborne pathogen, share highly homologous T6SS consisting of one large cluster and two small orphan or auxiliary clusters, and each of which was generally recognized as one operon. Here, we showed that the genes in each of the small clusters are organized into two transcriptional units. Specifically, the inner tube coding gene hcp/tssD is highly transcribed as one monocistron, while the tip component vgrG/tssI and its downstream effector and immunity genes are in one polycistron with very low transcriptional level. This conclusion is supported by qPCR analysis of mRNA abundance, reporter fusion analysis and transcriptional unit definition with RT-PCR analysis. Taking tssI2_a of V. fluvialis as an example, we further demonstrated that quorum sensing (QS) regulator HapR and global regulator IHF activate vgrG/tssI transcription by directly binding to its promoter region. Taken together, current studies deepen our understanding of T6SS system, highlighting its regulatory complexity during functional execution process.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Vibrio
/
Vibrio cholerae
/
Vibrio Infections
/
Gene Expression Regulation, Bacterial
/
Type VI Secretion Systems
Limits:
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2021
Document type:
Article
Affiliation country:
China