Preparation and evaluation of virus-like particle vaccine against H3N8 subtype equine influenza.
Microb Pathog
; 157: 104885, 2021 Aug.
Article
in En
| MEDLINE
| ID: mdl-33991641
ABSTRACT
To prevent and control H3N8 subtype equine influenza, we prepared virus-like particles (VLPs) comprising the HA, NA and M1 proteins of H3N8 equine influenza virus (EIV) through the insect cell-baculovirus expression system. The results of Western blot and hemagglutination analyses demonstrated that the constructed VLPs comprising HA, NA and M1 proteins have good hemagglutination activity. Immunoelectron microscope revealed that the VLPs share similar morphology and structure with natural virus particles. The hyperimmune serum from horses immunized with the VLPs were injected into mice by means of artificial passive immunization and then challenge, or challenge following by injecting hyperimmune serum. The results showed that the equine hyperimmune serum has good preventive and therapeutic efficacy against the infection of H3N8 EIV. The study provides a technical foundation for the development of H3N8 EIV VLP vaccine.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Influenza Vaccines
/
Orthomyxoviridae Infections
/
Influenza, Human
/
Influenza A Virus, H3N8 Subtype
/
Vaccines, Virus-Like Particle
/
Horse Diseases
Limits:
Animals
Language:
En
Journal:
Microb Pathog
Journal subject:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
China